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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in many tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two extra splice variants were being noted but in depth nucleotide sequence info is not nonetheless obtainable.

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Blocking peptides are peptides that bind specially to your concentrate on antibody and block antibody binding. These peptide ordinarily includes the epitope recognized through the antibody.

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This information outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best final results are...

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This information outlines additional probable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...

Antibodies bound to the blocking peptide no more bind to the epitope to the target protein. By comparing the staining through the blocked antibody vs . the antibody alone, one can see which staining is certain.

Focus on Information and facts Cyclin E belongs to the very conserved cyclin spouse and children, whose members are characterized more info by a dramatic periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which contribute for the temporal coordination of each mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development by way of S stage.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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